Idk if this is the right place to ask? But sprite help

[Glace Leau]

If you want to do it in Photoshop:

Duplicate your Background layer (the one with the spritesheet)
Delete the old Background layer
Use the magic wand
Uncheck Contiguous, make sure anti-alias is off and tolerance is 0
Select the background; it'll select everything that's the same color
Press delete